GlycoTechnica Ltd.

Products List

Products: Glycan Profiling Analysis Tools using Lectin Microarrays

Glycosylation is a common posttranslational modification of proteins where glycan chains are added to a protein molecule. Most of proteins are glycosylated resulting in changes in the protein such as folding, targeting, ligand recognition, binding, stability, immunogenicity, biological activity, etc.

Today, glycans play a significant biological role in four major medical areas; cancer, immunological disease, infectious disease and regenerative medicine. Glycoproteins show promise in the area of cancer biomarkers. Approximately 50% of current cancer markers widely used in clinical cancer diagnosis, are, in fact, various types of Glyco-markers. It is common knowledge in the infectious disease community that most communicable viruses target receptor glycoproteins formed on cell membranes. This fact provides drug developers with opportunities to develop vaccines directed at these target glycoproteins. These are only a few of the many examples that illustrate the need for a quick, simple and highly sensitive method for glycan profiling analysis which can then be used by the biopharmaceutical industry to accelerate the development of advanced biomarkers, thereapeutic proteins, stem cell (iPS, ES, and MSC) characterization, and also probiotics methodologies; all leading to improved diagnostics, therapeutic treatments, and health enhancement.

GlycoStation™ is a Gold Standard in Glycan Profilinglink to PDF

GlycoStation™ Evanescent-field Fluorescence Excitation Glycan Profiler

GlycoStation™ Reader 1200GlycoStation™ Reader 1200 is an ultra-high speed and highly sensitive optical microarray scanner utilizing the principle of evanescent-field fluorescence excitation to scan an entire microarray slide in only two minutes.

A successor model named "GlycoStation™ Reader 2200" was released in the end of June, 2016.

The scan time is to be greatly improved from 1min40sec to only 10sec at 1sec exposure time condition (Sept., 2017)







GlycoLite™ 2200, Evanescent-field Fluorescence Excitation Glycan Profiler

GlycoLite™ 2200GlycoLite™ 2200 is a low cost version of GlycoStation™ Reader 1200 based on the same principle of evanescent-field fluorescence excitation to scan LecChip Ver.1.0.

This product was released in Apr, 2017. The appearance and specifications are subject to change.






Comparative chart between GlycoStation™ Reader 2200 and GlycoLite™ 2200 is shown below.

GlycoStation™ Reader 2200 GlycoLite™ 2200
Scanning method Step & Repeat, sCMOS Step & Repeat, sCMOS
Exposure time 133msec - 10sec 133msec - 10sec
EMCCD gain none none
Excitation method Evanescent-field Excitation Evanescent-field Excitation
Liquid phase detection possible possible
Scanning area 13x65mm Standard, 24x65mm at the Maximum 13x65mm
Resolution 6.5 µm 6.5 µm
Sensitivity 250pg/mL 2ng/mL
Scanning time 10sec at 1sec Exposure Time 2min35sec at 1sec Exposure Time
Light source Metal halide lamp LED
Fluorescence wavelength Cy3, Cy5, FITC (up to 6) Cy3 monochromatic
Digital resolution 16bits 16bits
Analysis software(Option) GlycoStation ToolsPro GlycoStation ToolsPro
Data storage HDD, Exportable through LAN or USB HDD HDD, Exportable through LAN or USB Memory
Size 440x592x585mm 304x425x480mm
Weight 65kg 27kg

LecChip™ Ver. 1.0 Lectin Microarrays (Lectin Arrays)

LecChip™ Ver. 1.0 Lectin MicroarraysThe LecChip™ Ver 1.0 lectin microarrays feature 7 wells on a glass slide with 45 lectins in each well. Each lectin is spotted in triplicate.
Lectins immobilized on LecChips and its layoutlink to PDF








Differential Glycan Profiling Analysis Software:GlycoStation™ ToolsPro Ver.1.5

Differential Glycan Profiling Analysis Software:GlycoStation™ ToolsPro Ver.2.0

GlycoStation™ ToolsPro is an integrated glycan profiling analysis software which covers all of the necessary functions starting from digitizing fluorescence patterns and ending upto the function of differential profiling. GlycoStation™ ToolsPro Suite ver.2.0 is an updated version of ToolsPro ver.1.5. This software is able to digitize spot intensities using those local B/G values, so that the the accuracy is greatly improved even if there is some non-uniformity in the background.

Reagent optimized for LecChip™ Probing Solution

Dedicated Probing buffer Solution; Ver.1.0In order to maximize the S/N ratios of the glycan profiling analysis, it is highly recommended to use our proprietary probing solution for the lectin microarrays. This solution is not only for the preparation of sample solutions but also for washing the LecChip surface to remove a protective coating.







Glycan Profiling Analysis Service

We can do glycan profiling analysis services with receiving your samples. Please visit Contract Analysis Session to know this service in detail. As for application examples, please take a look at Application webpage.







Culture Media for Mesenchymal Stem Cells and EC cells

Culture MediaThere are four kinds of Culture Media. We can deal with your order from a bottle, of which volume is 250mL. Growth factors, antibiotics and serum (10%) are contained in these culture media, so that you can use these as is.

Products Name Designed for Price (in Japanese Yen)
Poweredby10 Mesenchymal Stem Cells DMEM 23,000 JPY
Plusoid-M Mesenchymal Stem Cells MSCBM 34,000 JPY
G031101 EC Cells 17,800 JPY
M061101 Mesenchymal Stem Cells 19,500 JPY

Shipping Schedule: Deliver by Thursday as ordered by the last Thursday
Lifetime: One month
Shipping and Storage Condition: cold storage, avoid freezing
Remarks) Freight fee is not included in the price.
These are recommended products by RIKEN-BRC and National Institute of Biomedical Innovation.

Culture Media for Human Hepatocytes

This product is only for R&D purpose.

Product Name Designed for Price(in Japanese Yen)
ESTEM™-5494 Human Hepatocytes 33,000 JPY(500mL), 19,500 JPY(250mL)

Shipping Schedule: Deliver by Thursday as ordered by the last Thursday
Lifetime: One month
Shipping and Storage Condition: cold storage, avoid freezing
Remarks) Freight fee is not included in the price.


Cutting-edge Culture Medium for iPS Cells "SODATT™" and a most easy to use Culture Medium ESTEM™-UX

Culture Media for iPSThere are three types of culture media for iPS. We can deal with your order from a bottle, of which volume is 500mL. SODATT™-E7 is a cutting-edge culture medium which fulfills Serum-free, Xeno-free, bFGF/TGFβ-free, and Feeder-freeconditions. This product is to be released in Sept, 2017. SODATT™-E8 is a standard culture medium for iPS utilizing feeder cells and is designed to meet Serum-free, Xeno-free requirements. Unlike SODATT™-E7, itis required to add bFGF in this product.

Product Name Designed for Price (in Japanese Yen)
SODATT™-E7 iPS 20,000 JPY
SODATT™-E8 iPS 25,000 JPY
ESTEM™-UX iPS 28,500 JPY

Shipping Schedule: Deliver by Thursday as ordered by the last Thursday
Lifetime: Usein two weeks after mixing a supliment.
Shipping and Storage Condition: cold storage for tha main bottle and freezingfor the supliment.
Remarks) Freight fee is not included in the price.

Remarks)
  1. ESTEM™-UX can not be used in a Feeder-free condition.
  2. Vitronectin is recommended in Feeder-free cultivation.
  3. It is recommended to add Y27632(ROCK Inhibitor) at a passage.
  4. There is no need to add bFGF/TGFβ in SODATT™-E7.
  5. It is needed to add bFGF/TGFβ in ESTEM™-UX.

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